Glucose Electrode Using Glassy Carbon as Base Electrode


Amperometric enzyme electrode for glucose based on collagen membrane was developed. The glucose oxidase was co-crosslinked with reconstructed collagen using the bifunctional agent, glutaraldehyde. The base electrode used was glassy carbon electrode. Membranes containing 20 units/cm^2 glucose oxidase and 0.05 mg/cm^2 glutaraldehyde gave the most preferable membrane characteristics. The optimum pH and the optimum temperature of the immobilized enzyme membrane was pH 6.6 and 35~40℃, respectively. The enzyme electrode gave the highest response and the best linearity at the potential of +0.9~+1.0 V (vs. Pt). The response time of the enzyme electrode was less than 1 min if a steady-state method was used. The response was proportional to glucose concentration in the 40 μM ~ 1 mM range. The recovery test gave 102 % in average. The enzyme electrode was stable for over a half-year. This electrode is very favorable for glucose assays in food samples.再生コラーゲン膜を用いたアンペロメトリック型グルコース電極を開発した.グルコースオキシダーゼを二官能試薬であるグルタルアルデヒドにより再生コラーゲン膜に架橋した.下地電極にはグラッシーカーボン電極を用いた.20units/cm^2のグルコースオキシダーゼおよび0.05mg/cm^2のグルタルアルデヒドを含む膜が最も良好な膜特性を与えた.固定化酵素の至適pHおよび至適温度はそれぞれpH6.6および35~40℃ であつた.本酵素電極は+0.9~+1.OV(vs.Pt)において最高の応答および最良の直線性を示した.定常法を用いた場合,酵素電極の応答時間は1分以下であつた。応答は40μM~1mMの領域のグルコースに対して直線を与えた。添加実験による回収率は平均102%であつた.試作酵素電極は半年以上にわたつて安定であつた.本酵素電極は食品試料のグルコース分析に有効と考える

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Kyushu University Institutional Repository

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