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Parent bisphenol A accumulation in the human maternal-fetal-placental unit.

By Gilbert Schönfelder, Werner Wittfoht, Hartmut Hopp, Chris E Talsness, Martin Paul and Ibrahim Chahoud


Bisphenol A (BPA), an endocrine disruptor, is employed in the manufacture of a wide range of consumer products. The suggestion that BPA, at amounts to which we are exposed, alters the reproductive organs of developing rodents has caused concern. At present, no information exists concerning the exposure of human pregnant women and their fetuses to BPA. We therefore investigated blood samples from mothers (n = 37) between weeks 32 and 41 of gestation. Afer the births, we also analyzed placental tissue and umbilical cord blood from the same subjects. We developed a novel chemical derivatization-gas chromatography/mass spectrometry method to analyze parent BPA at concentrations < 1 micro g/mL in plasma and tissues. Concentrations of BPA ranged from 0.3 to 18.9 ng/mL (median = 3.1 ng/mL) in maternal plasma, from 0.2 to 9.2 ng/mL (median = 2.3 ng/mL) in fetal plasma, and from 1.0 to 104.9 ng/g (median = 12.7 ng/g) in placental tissue. BPA blood concentrations were higher in male than in female fetuses. Here we demonstrate parent BPA in pregnant women and their fetuses. Exposure levels of parent BPA were found within a range typical of those used in recent animal studies and were shown to be toxic to reproductive organs of male and female offspring. We suggest that the range of BPA concentrations we measured may be related to sex differences in metabolization of parent BPA or variable maternal use of consumer products leaching BPA

Topics: Research Article
Year: 2002
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Provided by: PubMed Central

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  8. (2000). Disposition of orally administered 2,2-bis(4-hydroxyphenyl)propane (bisphenol A) in pregnant rats and the placental transfer to fetuses. Environ Health Perspect 108:931–935.
  9. (1996). Estrogenicity of resin-based composites and sealants used in dentistry. Environ Health Perspect 104:298–305.
  10. (1999). Exposure to bisphenol A advances puberty.
  11. (2001). Exposure to bisphenol A during the fetal and suckling periods disrupts sexual differentiation of the locus coeruleus and of behavior in the rat.
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  13. (2001). In utero exposure to bisphenol A alters the development and tissue organization of the mouse mammary gland.
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  15. (2001). In vitro and in vivo interactions of bisphenol A and its metabolite, bisphenol A glucuronide, with estrogen receptors alpha and beta. Chem Res Toxicol 14:149–157.
  16. (1999). Lack of effects for low dose levels of bisphenol A and diethylstilbestrol on the prostate gland of CF1 mice exposed in utero. Regul Toxicol Pharmacol 30:156–166.
  17. (1999). Low-dose bioactivity of xenoestrogens in animals: fetal exposure to low doses of methoxychlor and other xenoestrogens increases adult prostate size in mice. Toxicol Ind Health 15:12–25.
  18. (1998). Materials Flow Analysis of Major Endocrine Disrupting Industrial Chemicals (Bisphenol A; Dibutyl Phthalate/Benzyl Butyl Phthalate; Nonylphenol/ Alkylphenol Ethoxylates).
  19. (1999). Normal reproductive organ development in CF-1 mice following prenatal exposure to bisphenol A. Toxicol Sci 50:36–44.
  20. Normal reproductive organ development in Wistar rats exposed to bisphenol A in the drinking water. Regul Toxicol Pharmacol 30:130–139.
  21. (2000). Pharmacokinetics of bisphenol A released from a dental sealant.
  22. (2001). Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography-electrospray mass spectrometry.
  23. (1999). Quantities of bisphenol A leached from plastic waste samples.
  24. (1997). Relative binding affinity-serum modified access (RBA-SMA) assay predicts the relative in vivo bioactivity of the xenoestrogens bisphenol A and octylphenol. Environ Health Perspect 105:70–76.
  25. (2000). Royal society warns on hormone disrupters.
  26. (2001). Sensitive determination of bisphenol A in environmental water by gas chromatography with nitrogen-phosphorus detection after cyanomethylation.
  27. (2002). Serum bisphenol A concentrations showed gender differences, possibly linked to androgen levels.
  28. (1998). Sex and long-term soy diets affect the metabolism and excretion of soy isoflavones in humans.
  29. (1936). Synthetic oestrogenic agents without the phenanthrene nucleus.
  30. (2000). The effects of low and high doses of bisphenol A on the reproductive system of female and male rat offspring. Congenital Anom 40:S94–S107.
  31. (1988). The inadequacy of perinatal glucuronidation: immunoblot analysis of the developmental expression of individual UDP-glucuronosyltransferase isoenzymes in rat and human liver microsomes. Mol Pharmacol 34:729–735.
  32. (2000). The relative bioavailability and metabolism of bisphenol A in rats is dependent upon the route of administration.
  33. (2001). Trace organic contaminants in sediment and water from Ulsan Bay and its vicinity, Korea. Arch Environ Contam Toxicol 40:141–150.
  34. (1995). Xenoestrogens released from lacquer coatings in food cans. Environ Health Perspect 103:608–612.

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