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Field ecology of toxic Pfiesteria complex species and a conservative analysis of their role in estuarine fish kills.

By H B Glasgow, J M Burkholder, M A Mallin, N J Deamer-Melia and R E Reed


Within the past decade, toxic Pfiesteria outbreaks have been documented in poorly flushed, eutrophic areas of the largest and second largest estuaries on the U.S. mainland. Here we summarize a decadal field effort in fish kill assessment, encompassing kills related to Pfiesteria (49 major kills in North Carolina estuaries since 1991 and 4 in Maryland estuaries in 1997) and to other factors such as low oxygen stress (79 major fish kills in North Carolina estuaries). The laboratory and field data considered in developing our protocols are described, including toxic Pfiesteria behavior, environmental conditions conducive to toxic Pfiesteria activity, and impacts of toxic clonal Pfiesteria on fish health. We outline the steps of the standardized fish bioassay procedure that has been used since 1991 to diagnose whether actively toxic Pfiesteria was present during estuarine fish kills. Detailed data are given for a 1998 toxic Pfiesteria outbreak in the Neuse Estuary in North Carolina to illustrate of the full suite of diagnostic steps completed. We demonstrate that our conservative approach in implicating toxic Pfiesteria involvement in fish kills has biased in favor of causes other than Pfiesteria. Data are summarized from experiments that have shown stimulation of toxic Pfiesteria strains by nutrient (N, P) enrichment, supporting field observations of highest abundance of toxic strains in eutrophic estuaries. On the basis of a decade of research on toxic Pfiesteria, we present a conceptual model of the seasonal dynamics of toxic strains as affected by changing food resources and weather patterns. We also recommend protocols and research approaches that will strengthen the science of fish kill assessment related to Pfiesteria and/or other causative factors

Topics: Research Article
Year: 2001
OAI identifier:
Provided by: PubMed Central

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  1. (1993). A new ichthyotoxic dinoflagellate: cause of acute mortality in aquarium fishes.
  2. (2001). A new species of ichthyotoxic Pfiesteria (Dinamoebales,
  3. (1984). An investigation of surface drift on the Labrador Coast. Canada Technical Report
  4. (1999). Assessing the relationship between Pfiesteria and estuarine fish kills.
  5. (1993). Assessing water quality with a submersed macrophyte.
  6. (2000). Associations Between Pfiesteria, Fish Health, and Environmental Conditions in Maryland. Final Report. Annapolis, MD:Maryland Department of Natural Resources,
  7. (1992). Behavioral response of fish larvae to low dissolved oxygen concentrations in a stratified water column.
  8. (1998). Bloom dynamics and physiology of domoic-acid-producing Pseudo-nitzschia species. In:
  9. (1998). Bloom dynamics and physiology of Prymnesium and Chrysochromulina. In:
  10. (1976). Causation and disease: the Henle-Koch postulates revisited.
  11. (1986). Characterization of the North Carolina Pamlico-Albemarle Estuarine Complex.
  12. (2000). Comparative culture and toxicity studies between the toxic dinoflagellate, Pfiesteria piscicida and a morphologically similar cryptoperidiniopsoid dinoflagellate.
  13. (2001). Current progress in isolation and characterization of toxins isolated from Pfiesteria piscicida. Environ Health Perspect 109(suppl 5):739–743
  14. (1996). Department of Environment, Health & Natural Resources [NCDEHNR]. Classifications and Water Quality Standards Applicable to Surface Waters of North Carolina.
  15. (1990). Descriptive Physical Oceanography: An Introduction.
  16. (1998). Diagnostic assessment of non-infectious disorders of captive and wild fish populations and the use of fish as sentinel organisms for environmental studies. In:
  17. (1995). Discovery of the “phantom” dinoflagellate in Chesapeake Bay.
  18. (1993). Distribution and abundance of larval fishes at two North Carolina inlets. Estuar Coast Shelf Sci 37:161–179
  19. (1995). Distribution and environmental conditions for fish kills linked to a toxic ambushpredator dinoflagellate. Mar Ecol Prog Ser
  20. E. Discovery of the “phantom” dinoflagellate in Chesapeake Bay.
  21. (1998). Ecosystem responses to internal and watershed organic matter loading: consequences for hypoxia in the eutrophying Neuse River Estuary,
  22. (1990). Effects of fish chemical cues on vertical migration behavior of Chaoborus predaton on zooplankton and the calculation of relative prey vulnerabilities.
  23. (2000). Effects of hypoxia on movements and behavior of selected estuarine organisms from the southeastern United States.
  24. (1991). Effects of periodic hypoxia on distribution of demersal fish and crustaceans.
  25. (1995). Effects of small-scale turbulence on photosynthesis, pigmentation, cell division, and cell size in the marine dinoflagellate Gonyaulax polyedra (Dinophyceae).
  26. (1998). Effects of turbulence on phytoplankton. In: Physiological Ecology of Harmful Algae (Anderson DM,
  27. (2000). Enzymatic biomarker measurement and study of DNA adduct formation in benzo[a]pyrene-contaminated mussels, Mytilus galloprovincialis.
  28. (1990). Exotoxin production by Gyrodinium cf. aureolum (Dinophyceae).
  29. (1997). Feeding behavior of the ichthyotoxic estuarine dinoflagellate, Pfiesteria piscicida, on amino acids, algal prey, and fish vs mammalian erythrocytes. In: Harmful Microalgae (Reguera
  30. Field Manual for the Investigation of Fish Kills.
  31. (1999). Fish kills, bottomwater hypoxia, and the toxic Pfiesteria complex in the Neuse River and Estuary. Mar Ecol Prog Ser
  32. (1988). Fisherman’s Guide - Fishes of the Southeastern United States. Raleigh, NC:North Carolina Museum of Natural History,
  33. (1997). General methodology – cultivation of microbial consortia and communities. In:
  34. (1997). Habitat and Biological Conditions of River Systems Affected by Pfiesteria or Pfiesteria-Like Organisms on the Lower Eastern Shore of Maryland:
  35. (2000). Heteroduplex mobility assay guided sequence discovery: elucidation of the small subunit (18S) rDNA sequence of Pfiesteria piscicida from complex algal culture and environmental sample DNA pools.
  36. (2001). Identification of a P2X7 receptor in GH4C1 rat pituitary cells: a target for a bioactive substance produced by Pfiesteria piscicida.
  37. (1984). III. Effects of chaetognath predation and nutrient enrichment on enclosed estuarine copepod communities.
  38. (1997). Impacts to a coastal river and estuary from rupture of a large swine waste holding lagoon.
  39. (1998). Implications of harmful marine microalgae and heterotrophic dinoflagellates in management of sustainable marine fisheries.
  40. (1995). Insidious effects of a toxic dinoflagellate on fish survival and human health.
  41. (2000). Interactions Between Two Commercially Important Species of Bivalve Molluscs and the Toxic Estuarine Dinoflagellate, Pfiesteria piscicida [MS Thesis].
  42. (1995). Interactions of a toxic estuarine dinoflagellate with microbial predators and prey.
  43. (1999). Kleptoplastidy in the toxic dinoflagellate Pfiesteria piscicida.
  44. (1992). Koch’s postulates and the etiology of AIDS: an historical perspective. Publ Stn Zool
  45. Maryland Guidelines for Closing and Reopening Rivers Potentially Affected by Pfiesteria or Pfiesteria-Like Events. Annapolis, MD:Office of the Governor,
  46. (1999). Mixotrophy and nitrogen uptake by Pfiesteria piscicida (Dinophyceae).
  47. (1988). Mortality in Tilapia aurea due to a toxic dinoflagellate bloom. In:
  48. (1991). Multi-year distribution patterns of nutrients within the Neuse River Estuary, North Carolina. Mar Ecol Prog Ser
  49. (2000). National Coastal Assessment – Coastal
  50. National Oceanic & Atmospheric Administration [NOAA]. Pfiesteria Monitoring Report – NOAA’s Workshops to Standardize Pfiesteria Monitoring (Dec. ’98) and to Standardize Fish Health Monitoring Protocols
  51. (1992). New “phantom” dinoflagellate is the causative agent of major estuarine fish kills.
  52. (1996). NOAA’s Estuarine Eutrophication Survey. Vol 1: Southern Atlantic Region. Silver Spring,
  53. (2000). North and South Carolina coasts.
  54. (1996). Novel toxic dinoflagellate causes epidemic disease in estuarine fish.
  55. (1992). Nutritional strategies in dinoflagellates—a review with emphasis on cell biological aspects.
  56. (2001). Overview and present status of the toxic Pfiesteria complex.
  57. (1999). PCR and FISH detection extends the range of Pfiesteria piscicida in estuarine waters.
  58. (1997). Persistent learning deficits in rats after exposure to Pfiesteria piscicida.
  59. (1996). Pfiesteria piscicida (Pfiesteriaceae, fam. nov.), a new toxic dinoflagellate with a complex life cycle and behavior.
  60. (1997). Pfiesteria piscicida and other toxic Pfiesteria-like dinoflagellates: behavior, impacts, and environmental controls.
  61. (1999). Pfiesteria toxin and learning performance.
  62. (2000). Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates.
  63. (1998). Physiological ecology of Pfiesteria piscicida with general comments on “ambushpredator” dinoflagellates. In:
  64. (1994). Phytoplankton ecology of North Carolina estuaries.
  65. (1985). Planktivore functional groups and patterns of prey selection in pelagic communities.
  66. (1997). Population Dynamics of Pfiesteria-Like Dinoflagellates,
  67. (1998). Problems in learning and memory occur in persons with environmental exposure to waterways containing toxin-producing Pfiesteria or Pfiesteria-like dinoflagellates.
  68. Rapid neurobehavioral analysis of Pfiesteria piscicida effects in juvenile and adult rats.
  69. (1999). Reporter gene assay for fish killing activity produced by Pfiesteria piscicida.
  70. (1977). Resource competition between planktonic algae: an experimental and theoretical approach.
  71. Responses of phytoplankton and pfiesteria-like dinoflagellate zoospores to nutrient enrichment in the Neuse River Estuary,
  72. (2001). Species of the toxic Pfiesteria complex and the importance of functional type in data interpretation. Environ Health Perspect 109(suppl 5):667–679
  73. (1998). Summary of Continuously Monitored Water Quality Data – Neuse River Estuary,
  74. Surface Drifter Study –
  75. (1993). Taxonomy of toxic Cyanophyceae (cyanobacteria). In:
  76. (2000). The fish-killing activity and nutrient stimulation of a second toxic Pfiesteria species. In:
  77. (1989). The Fresh and Salt Water Fishes of the World. New York:Alfred
  78. (1995). The impact of harmful algal blooms on finfish: occurrence of fish kills, pathology, toxicological mechanisms, ecological and economic impacts—a review. Plouzané:Institut Francais’ de Recherché por l’ Exploitation de la Mer,
  79. (1999). The lurking perils of Pfiesteria.
  80. (2001). The standardized fish bioassay procedure for detecting and culturing actively toxic Pfiesteria, developed by two reference laboratories for Atlantic and Gulf coast states. Environ Health Perspect 109(suppl 5):745–756
  81. (1989). Toxin variability in Alexandrium. In:
  82. (1996). United States Environmental Protection Agency Environmental Investigations Standard Operating Procedures and Quality Assurance Manual. Athens:U.S. Environmental Protection Agency,
  83. Unpublished histopathology research of our laboratory with various fish pathologists.
  84. Unpublished histopathology research with our laboratory of fish gill following acute exposure to a mixed culture of actively toxic
  85. (2001). Use of molecular probes to assess geographic distribution of Pfiesteria species. Environ Health Perspect 109(suppl 5):765–767
  86. (2000). Utilization of PCR and FISH to Determine Fine Scale and Global Distribution of Pfiesteria Species [MS Thesis]. Greensboro, NC:University of North Carolina-Greensboro,
  87. (2000). Water quality trends and management implications from a five-year study of a eutrophic estuary.

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