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Organophosphorous pesticide exposure increases the frequency of sperm sex null aneuploidy.

By R Recio, W A Robbins, V Borja-Aburto, J Morán-Martínez, J R Froines, R M Hernández and M E Cebrián


It has been estimated that 4 of 1,000 live births and 35% of spontaneous abortions are aneuploid and that an important proportion of embryo and newborn aneuploidy is of paternal origin. Exposure to organophosphorous pesticides (OP) has been associated with sperm hyperploidy/polyploidy. Therefore, we aimed to assess the frequency of sperm aneuploidy (X, Y, and 18) and its relationship with urinary OP metabolites in agricultural workers. We performed multicolor fluorescence in situ hybridization on samples from nine men obtained before and during the pesticide spraying season to assess sperm aneuploidy. We measured urinary OP metabolite levels by gas-liquid chromatography. Aneuploidies were found in 0.67% of total sperm nuclei. The most frequent aneuploidy was the lack of a sexual chromosome or sex null (0.19%), followed by XY18 (0.15%) and XY18-18 (0.06%). OP metabolites detected at higher concentrations were dimethylthiophosphate, dimethyldithiophosphate, and diethylphosphate (DEP). There were no differences in average aneuploidy frequency or urinary metabolite levels between samples collected before and after exposure. However, Poisson regression analysis adjusted for age, alcohol intake, and sperm concentration showed significant associations between OP metabolite concentrations and increased frequency of sperm aneuploidies. The association was more evident between DEP and sex null, and the risk increased further during the spraying season. Thus, OP exposure could interfere with sperm chromosome segregation and increase the risk for genetic syndromes, such as Turner's. Further studies are required to assess the prevalence of spontaneous abortions, birth defects, and genetic syndromes in agricultural communities

Topics: Research Article
Year: 2001
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Provided by: PubMed Central

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  5. (1996). Aneuploidy in germ cells: disruption of chromosome mover components.
  6. (1996). Aneuploidy in human sperm: the use of multicolor FISH to test various theories of nondisjunction.
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  9. (1998). Donor age and the frequency of disomy for chromosomes 1,13,21 and structural abnormalities in human spermatozoa using multicolour fluorescence in-situ hybridisation.
  10. Generalized linear models. In:
  11. (1995). Hassold TJ. Non-disjunction in human sperm: evidence for an effect of increasing paternal age.
  12. (1996). Human aneuploidy: incidence, origin and aetiology.
  13. (1998). Human sperm chromosome complements in chemotherapy patients and infertile men.
  14. (1999). Improved cleanup and determination of dialkylphsophates in the urine of children exposed to organophosphorous insecticides.
  15. (1997). Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization.
  16. (1980). Manual of Analytical Methods for the Analysis of Pesticides in Humans and Environmental Samples;
  17. (1994). Mechanism of non-disjunction in mammalian meiosis.
  18. (1996). Meiotic recombination and germ cell aneuploidy.
  19. (1992). Methyl parathion-induced sperm shape abnormalities in mouse.
  20. (1996). Multicolor fluorescence in situ hybridization analysis of aneuploidy and diploidy frequencies in 225,846 sperm from 10 normal men.
  21. (1999). Numerical and structural chromosomal abnormalities detected in human sperm with a combination of multicolor FISH assays.
  22. (1992). Organization. WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction.
  23. (1999). Sperm aneuploidy among Chinese pesticide factory workers: scoring by the FISH method.
  24. (1998). Spermatozoa with chromosomal abnormalities may result in a higher rate of recurrent abortion.
  25. (1992). The chromosome complement of human gametes. In:
  26. (1997). Use of fluorescence in situ hybridisation (FISH) to assess effects of smoking, caffeine, and alcohol on aneuploidy load in sperm of healthy men.
  27. (1997). Wyrobek AJ. Chemotherapy induces transient chromosomal and autosomal aneuploidy in human sperm.
  28. (1998). Wyrobek AJ. Smoking cigarettes is associated with increased sperm disomy in teenage men.

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