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The effect of matrix on the binding of albumin to immobilized Cibacron Blue.

By S Angal and P D Dean

Abstract

With Cibacron Blue as a ligand, the suitability of Sepharose, Sephacryl, cellulose and Ultrogel as column supports for the affinity chromatography of human serum albumin was examined. Sepharose and Sephacryl were the most effective matrices. Albumin was readily removed from such columns by using an electrophoretic desorption technique

Topics: Research Article
Year: 1977
DOI identifier: 10.1042/bj1670301
OAI identifier: oai:pubmedcentral.nih.gov:1183653
Provided by: PubMed Central
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