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An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol.

By J M Stein, G A Smith and J P Luzio

Abstract

A method for the quantification of membrane lipids by acetylation with [3H]acetic anhydride is described. A standard lipid, labelled with 14C or 32P, is added to the sample and is simultaneously acetylated. The 3H/14C or 3H/32P ratios obtained in the acetylated lipids are proportional to the initial lipid concentration. The method has been used to quantify cholesterol, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in amounts between 2 and 10 nmol

Topics: Research Article
Year: 1991
DOI identifier: 10.1042/bj2740375
OAI identifier: oai:pubmedcentral.nih.gov:1150147
Provided by: PubMed Central
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