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Production of recombinant sarcotoxin IA in Bombyx mori cells.

By K Yamada, Y Nakajima and S Natori

Abstract

A cDNA for sarcotoxin IA, an antibacterial protein of Sarcophaga peregrina (fleshfly), was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a line of Bombyx mori cells. When a cysteine proteinase inhibitor, p-chloromercuribenzenesulphonic acid, was present in the culture medium, a significant amount of recombinant sarcotoxin IA accumulated, but without this reagent the product seemed to be degraded in this system. The C-terminus of the recombinant sarcotoxin IA seemed to be glycine, not amidated arginine as found in authentic sarcotoxin IA. Probably, Bm-N cells lack the C-terminal alpha-amidation enzyme

Topics: Research Article
Year: 1990
DOI identifier: 10.1042/bj2720633
OAI identifier: oai:pubmedcentral.nih.gov:1149755
Provided by: PubMed Central
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