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Circular dichroism and fluorescence investigations on Vicia faba lectin-saccharide binding.

By P K Datta, P S Basu and T K Datta

Abstract

Vicia faba lectin contained 40-57% beta-conformation, 4-23% alpha-conformation along with random coil at pH 7.2 depending upon the analytical methods used. The percentage of beta-conformation increased with the addition of N-acetyl-D-glucosamine or methyl alpha-D-mannopyranoside. The structural transitions of V. faba lectin were affected by alkali at pH 9.6 and 10.6. Binding constants and free energy changes for the interaction between V. faba lectin and N-acetyl-D-glucosamine and methyl alpha-D-mannopyranoside were estimated at pH 7.2 using the c.d. and fluorescence methods

Topics: Research Article
Year: 1988
DOI identifier: 10.1042/bj2510195
OAI identifier: oai:pubmedcentral.nih.gov:1148983
Provided by: PubMed Central
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