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A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.

By R D Harper

Abstract

Perifused fat-cells showed similar values for acylglycerol glycerol synthesis from glucose with insulin and for the effects of added palmitate to those in normal incubations and those reported in the literature. Fatty acid synthesis was lower in perifused cells compared with normal incubations, and there was a net release of fatty acids only with the perifused fat-cells. Hence fluxes of metabolites were different in the two incubation systems, and the perifusion system enables the investigation of the flux of metabolites under conditions which may more closely resemble those in vivo

Topics: Research Article
Year: 1984
DOI identifier: 10.1042/bj2240235
OAI identifier: oai:pubmedcentral.nih.gov:1144418
Provided by: PubMed Central
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