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Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae

By Pramod K. Rompikuntal, Svitlana Vdovikova, Marylise Duperthuy, Tanya L. Johnson, Monika Åhlund, Richard Lundmark, Jan Oscarsson, Maria Sandkvist, Bernt Eric Uhlin and Sun Nyunt Wai

Abstract

Background Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. Methodology/Principal Findings In this study, we demonstrated that PrtV was secreted from the wild type V. cholerae strain C6706 via the type II secretion system in association with OMVs. By immunoblotting and electron microscopic analysis using immunogold labeling, the association of PrtV with OMVs was examined. We demonstrated that OMV-associated PrtV was biologically active by showing altered morphology and detachment of cells when the human ileocecum carcinoma (HCT8) cells were treated with OMVs from the wild type V. cholerae strain C6706 whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Furthermore, OMV-associated PrtV protease showed a contribution to bacterial resistance towards the antimicrobial peptide LL-37. Conclusion/Significance Our findings suggest that OMVs released from V. cholerae can deliver a processed, biologically active form of PrtV that contributes to bacterial interactions with target host cells

Topics: Other Basic Medicine, Annan medicinsk grundvetenskap
Publisher: 'Public Library of Science (PLoS)'
Year: 2015
DOI identifier: 10.1371/journal.pone.0134098
OAI identifier: oai:DiVA.org:umu-107868
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