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Stabilization process in Saccharomyces intra- and interspecific hybrids in fermentative conditions

By Laura Pérez-Través, Christian A. Lopes, Eladio Barrio and Amparo Querol

Abstract

© 2015 Sociedad Española de Microbiologia. All rights reserved. We evaluated the genetic stabilization of artificial intra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae × S. kudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome size and genome reorganizations were observed during this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids. The largest number of molecular patterns recovered among the derived clones was observed for intraspecific hybrids, particularly for those obtained by rare-mating. Molecular marker analyses revealed that unstable clones could change during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes in genetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30–50 generations) would suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.This work has been supported by grants AGL2009-12673-CO2-01 and AGL2009-12673-CO2-02 and AGL2012-39937-C02-01 and AGL2012-39937-C02-02 from the Spanish Government to A. Querol and E. Barrio, respectively. L. P-T. and C. L. wish to acknowledge the CSIC and the Spanish Ministry of Education and Science for an I3P fellowship and a postdoctoral contract, respectivelyPeer Reviewe

Publisher: Sociedad Española de Microbiología
Year: 2015
DOI identifier: 10.2436/20.1501.01.224
OAI identifier: oai:digital.csic.es:10261/122250
Provided by: Digital.CSIC
Journal:

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