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New application of air-dry techniques for studying Ephemeroptera and Plecoptera eggs by Scanning Electron Microscopy

By N. Ubero-Pascal, José Manuel Fortuño Alós and Mariàngels Puig

Abstract

8 páginas, 6 figuras.Hexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air-drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical point drying, but which involve a very simple process that saves time and money. Both techniques were applied to SEM studies of Ephemeroptera and Plecoptera eggs in order to assess their suitability as alternative methods to critical point drying for these kinds of biological material. The results show no morphological differences between eggs HMDS air-dried and critical point-dried.Peer reviewe

Topics: SEM, Hexamethyl-disilizane, Egg morphology, Air-drying techniques, Critical point drying, Tetramethylsilane
Publisher: 'Wiley'
Year: 2005
DOI identifier: 10.1002/jemt.20248
OAI identifier: oai:digital.csic.es:10261/40443
Provided by: Digital.CSIC
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