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IL-2 and IL-15 receptor α-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells

By György Vámosi, Andrea Bodnár, György Vereb, Attila Jenei, Carolyn K. Goldman, Jörg Langowski, Katalin Tóth, László Mátyus, János Szöllösi, Thomas A. Waldmann and Sándor Damjanovich

Abstract

The private α-chains of IL-2 and IL-15 receptors (IL-2R and IL-15R) share the signaling β- and γ(c)-subunits, resulting in both common and contrasting roles of IL-2 and IL-15 in T cell function. Knowledge of the cytokine-dependent subunit assembly is indispensable for understanding the paradox of distinct signaling capacities. By using fluorescence resonance energy transfer and confocal microscopy, we have shown that IL-2Rα, IL-15Rα, IL-2/15Rβ and γ(c)-subunits, as well as MHC class I and II glycoproteins formed supramolecular receptor clusters in lipid rafts of the T lymphoma line Kit 225 FT7.10. Fluorescence crosscorrelation microscopy demonstrated the comobility of IL-15Rα with IL-2Rα and MHC class I. A model was generated for subunit switching between IL-2Rα and IL-15Rα upon the binding of the appropriate cytokine resulting in the formation of high-affinity heterotrimeric receptors. This model suggests a direct role for the α-subunits, to which no definite function has been assigned so far, in tuning cellular responses to IL-2 or IL-15. In addition, both α-chains were at least partially homodimerized/oligomerized, which could be the basis of distinct signaling pathways by the two cytokines

Topics: Biological Sciences
Publisher: National Academy of Sciences
Year: 2004
DOI identifier: 10.1073/pnas.0403916101
OAI identifier: oai:pubmedcentral.nih.gov:503744
Provided by: PubMed Central
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