The absence of a B allele in acquired B blood group phenotype confirmed by a DNA based genotyping method.
A case of an 87 year old woman with carcinomatous sigmoid colon, found to be of group A1 with acquired B status, is reported. The acquired B phenotype was confirmed by the absence of a B allele in the patient using denaturing gradient gel electrophoresis of a DNA fragment amplified from the ABO locus by the polymerase chain reaction
DOI identifier: 10.1136/jcp.49.2.180
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