Article thumbnail

Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy

By Daryan Kempe, Antonie Schöne, Jörg Fitter and Matteo Gabba

Abstract

Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies

Topics: info:eu-repo/classification/ddc/530
Publisher: Soc.
Year: 2015
DOI identifier: 10.1021/acs.jpcb.5b02170
OAI identifier: oai:juser.fz-juelich.de:188896
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://juser.fz-juelich.de/rec... (external link)
  • http://juser.fz-juelich.de/sea... (external link)
  • http://juser.fz-juelich.de/rec... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.