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The Effect of Bicarbonate on Photosynthetic Oxygen Evolution in Flashing Light in Chloroplast Fragments

By Alan Stemler, Gerald T. Babcock and Govindjee

Abstract

The ability of bicarbonate ion (HCO(3)(-)) to stimulate photosynthetic oxygen evolution in maize chloroplast fragments exposed to continuous light depends on light intensity. Stimulation by HCO(3)(-) is less at low intensities. In HCO(3)(-)-depleted chloroplasts exposed to brief saturating light flashes, period 4 oscillations (in O(2) yield per flash) are damped within three cycles. Readdition of HCO(3)(-) to these preparations restores the oscillatory pattern to higher flash numbers, indicating that HCO(3)(-) reduces the probability of “misses” in the photosystem II reaction center. The rate of the dark relaxation reaction S(n)(′) → S(n+1) (where S refers to the oxidation state of the oxygen-evolving mechanism and n = 0, 1, or 2), after a photoact in the photosystem II reaction center, is retarded in HCO(3)(-)-depleted chloroplasts compared to the rate for this reaction in depleted chloroplasts to which HCO(3)(-) has been resupplied. However, the final oxygen-evolving reaction after the accumulation of four positive charges appears to be independent of HCO(3)(-). Bicarbonate has no effect on the dark deactivation of the higher oxidation states (S(2) and S(3)) of the positive charge-accumulating system. We propose two alternate ways in which the kinetic model of oxygen evolution developed by Kok et al. [(1970) Photochem. Photobiol. 11, 457-475] can be extended to include the action of HCO(3)(-)

Topics: Biological Sciences: Biophysics
Year: 1974
DOI identifier: 10.1073/pnas.71.12.4679
OAI identifier: oai:pubmedcentral.nih.gov:433959
Provided by: PubMed Central
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