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Nucleotide Sequence Analysis of RNA Synthesized from Rabbit Globin Complementary DNA

By Raymond Poon, Gary V. Paddock, Howard Heindell, Philip Whitcome, Winston Salser, Dan Kacian, Arthur Bank, Roberto Gambino and Francesco Ramirez

Abstract

Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of (32)P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T(1) and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the α or β globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants

Topics: Biological Sciences: Biochemistry
Year: 1974
DOI identifier: 10.1073/pnas.71.9.3502
OAI identifier: oai:pubmedcentral.nih.gov:433802
Provided by: PubMed Central
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