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Effect of Cerulenin on Growth and Lipid Metabolism of Mycoplasmas

By S. Rottem and M. F. Barile

Abstract

Cerulenin markedly inhibited the growth of Acholeplasma laidlawii. A. axanthum and A. granularum were less susceptible, whereas the sterol-requiring Mycoplasma species examined showed very little susceptibility. The inhibition was not reversed by the addition of long-chain fatty acids to the medium. At a concentration of 20 μg/ml, cerulenin inhibited the incorporation of [(14)C]acetate into A. laidlawii membrane lipids, but it had no effect on either protein or nucleic acid biosynthesis. Cerulenin inhibited both the de novo synthesis of long-chain fatty acids and the elongation of medium-chain fatty acids. As a result, carotenoid biosynthesis was stimulated, and increased amounts of oleic and elaidic acids were incorporated into membrane polar lipids. Our studies support the concept that cerulenin can serve as a useful tool for obtaining better control of fatty acid composition of A. laidlawii membranes

Topics: Chemistry; Mechanisms of Action and Resistance
Year: 1976
DOI identifier: 10.1128/aac.9.2.301
OAI identifier: oai:pubmedcentral.nih.gov:429518
Provided by: PubMed Central
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