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Intrasomatic Injection of Radioactive Precursors for Studying Transmitter Synthesis in Identified Neurons of Aplysia californica

By Michael Eisenstadt, James E. Goldman, Eric R. Kandel, H. Koike, John Koester and James H. Schwartz

Abstract

We introduced radioactive precursors directly into identified neurons of Aplysia californica. [(3)H]-Choline and L-[(3)H]tryptophan were injected with pressure into nerve cell bodies to study synthesis of acetylcholine and serotonin. We confirmed the cholinergic nature of R2, L10, and L11, identified neurons of the abdominal ganglion. Cells in the LD cluster (which contains motor neurons to the heart and gill) also converted most of the injected choline into acetylcholine. Neurons in the RB cluster (which contains an excitatory motor neuron to the heart) and the two metacerebral cells of the cerebral ganglion converted injected tryptophan to serotonin. No cell studied could convert both choline to acetylcholine and tryptophan to serotonin. Pressure permits rapid injection of precursors, from small amounts to amounts large enough to saturate intracellular synthetic pathways. In contrast to the results with injection, we found far less synthesis of acetylcholine and serotonin in identified nerve cell bodies when ganglia were incubated in the presence of the radioactive precursors

Topics: Biological Sciences: Cell Biology
Year: 1973
DOI identifier: 10.1073/pnas.70.12.3371
OAI identifier: oai:pubmedcentral.nih.gov:427239
Provided by: PubMed Central
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