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Studies on Purified Eel Acetylcholine Receptor and Anti-Acetylcholine Receptor Antibody

By Jim Patrick, Jon Lindstrom, Bill Culp and Jerri Mcmillan


Rabbit antiserum against purified Electrophorus electricus acetylcholine receptor is studied using an immunoprecipitin assay to measure either antibody titer or concentration of toxin-binding sites in solubilized receptor preparations. This antiserum, unlike control serum, blocks the electrophysiological response of the electroplax to carbamylcholine. Toxin (α-neurotoxin, Naja naja) and several cholinergic ligands produce partial inhibition of the reaction of antiserum with purified acetylcholine receptor. Evidence is presented that some of the toxin-binding sites on receptor, purified by affinity chromatography on toxin-agarose conjugates, are occluded by toxin. In addition, evidence is presented that antireceptor antiserum will cause precipitation of more toxin-binding sites present in an initial extract than in purified receptor preparations

Topics: Biological Sciences: Physiology
Year: 1973
DOI identifier: 10.1073/pnas.70.12.3334
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Provided by: PubMed Central
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