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Properties of the Enterotoxic Component in Escherichia coli Enteropathogenic for Swine

By Thomas M. Jacks, Bo J. Wu, Allen C. Braemer and Donald E. Bidlack

Abstract

The enterotoxic component in sterile syncase broth filtrates of Escherichia coli strains 340 (O9:K·:NM) and P307 (O8:K87,88a,b:H19) was studied. The enterotoxic activity in both strains was retained by an ultrafiltration membrane with a molecular weight retention of 100,000 (XM-100A) and eluted from a Sephadex G-200 column in the void volume. The enterotoxic activity in strain 340 was resistant to heating at 75 C for 30 min, but the activity in strain P307 was destroyed by heating at 60 C for 30 min. The P307 Sephadex G-200 column eluate possessing the enterotoxic activity, when desalted, contained 45.8% carbohydrate and 9.3% protein, and had an ED(50) of 2.2 mg/rabbit ileal loop. Immunodiffusion studies showed that this material contained both endotoxin and acid-polysaccharide capsular material. The enterotoxic activity was acidlabile and was destroyed by Pronase, but was resistant to trypsin and eluted as a single peak in the void volume of a 4% agarose column. The enterotoxic component could not be separated from the endotoxin; in fact, the data indicated that the two components are closely associated and that the enterotoxic activity resides in material of a protein nature

Topics: Pathogenic Mechanisms, Ecology, and Epidemiology
Year: 1973
OAI identifier: oai:pubmedcentral.nih.gov:422657
Provided by: PubMed Central
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