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MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana

By Robert Grant-Downton, Gael Le Trionnaire, Ralf Schmid, Josefina Rodriguez-Enriquez, Said Hafidh, Saher Mehdi, David Twell and Hugh Dickinson


New generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male gametophyte - is the specialised haploid structure that generates and delivers the sperm cells to the female gametes at fertilisation. Whether development and differentiation of the male gametophyte depends on the action of microRNAs and trans-acting siRNAs guiding changes in gene expression is largely unknown. Here we have used 454 sequencing to survey the various small RNA populations present in mature pollen of Arabidopsis thaliana.status: publishe

Topics: Arabidopsis, Base Sequence, Computational Biology, Gene Expression Regulation, Plant, MicroRNAs, Molecular Sequence Data, Nucleic Acid Conformation, Pollen, RNA, Plant, RNA, Small Interfering, Sequence Analysis, RNA
Publisher: BioMed Central
Year: 2009
OAI identifier:
Provided by: Lirias
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