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Purification of Pseudomonas aeruginosa Endotoxin by Membrane Partition Chromatography

By Nazario Rubio and Rubens Lopez


A procedure is described for obtaining large quantities of purified endotoxin of Pseudomonas aeruginosa by using Diaflo ultrafiltration. This method allowed us to isolate from the protein-lipopolysaccharide complex two low-molecular-weight substances which do not play any antigenic role. It provides a useful tool for immunological purposes

Topics: Clinical Microbiology, Virology, and Immunology
Year: 1972
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Provided by: PubMed Central
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