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High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.

By T Xie, S L Ho and O C Ma

Abstract

Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments

Topics: Research Article
Year: 1997
DOI identifier: 10.1136/mp.50.5.276
OAI identifier: oai:pubmedcentral.nih.gov:379648
Provided by: PubMed Central
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