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Preparative Electrophoresis of Isotopically Labeled L-Cell Interferons

By Daniel Stanček and Kurt Paucker

Abstract

The preparative method of polyacrylamide gel electrophoresis was adapted for purification and characterization of isotopically labeled L-cell interferons. Re-covery of interferon activity was quantitative, and purification and resolution were comparable to those obtained by analytical polyacrylamide gel electrophoresis. Ultimate specific activities attainable ranged from 2 × 10(6) to 3 × 10(6) international units per mg of protein

Topics: Clinical Microbiology, Virology, and Immunology
Year: 1971
OAI identifier: oai:pubmedcentral.nih.gov:377345
Provided by: PubMed Central
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