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DNA Synthesis by RNA-Containing Tumor Viruses

By Edward M. Scolnick, Stuart A. Aaronson and George J. Todaro

Abstract

Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties

Topics: Biological Sciences: Biochemistry
Year: 1970
OAI identifier: oai:pubmedcentral.nih.gov:283309
Provided by: PubMed Central
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