Location of Repository

Synthetic Spectroscopic Models Related to Coenzymes and Base Pairs, VII. Stacking Interactions in tRNA; the „Bend” at Dimethylguanosine*†

By Hajime Iwamura, Nelson J. Leonard and Josef Eisinger

Abstract

We have examined the stacking interactions of N2-dimethyl-guanosine with the nucleosides, e.g., adenosine and cytidine, found adjacent to it in certain tRNA's, by the use of model compounds in which the trimethylene bridge was substituted for the ribose-phosphate-ribose linkage. From the hypochromism exhibited by synthetic 9-[3-(aden-9-yl)propyl]-2-dimethylaminopurine-6-one (IV) and by 9-[3-(cytos-1-yl)propyl]2-dimethylaminopurin-6-one in aqueous solution (VI) it is appearent that the interaction is at least as great between the N2-dimethylguanine moiety and adenine or cytosine as between guanine and these two bases. The fluorescence and phosphorescence emission spectra were obtained in ethylene glycol-water glass at 80°K. The exciplex fluorescence observed for both bi-molecules (IV and VI) containing the N2-dimethylguanine unit provides further evidence for stacked chromophores

Topics: Biological Sciences: Biochemistry
Year: 1970
OAI identifier: oai:pubmedcentral.nih.gov:283018
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.