Skip to main content
Article thumbnail
Location of Repository

Functional reconstitution of a proton-translocating system responsive to fusicoccin.

By P Aducci, A Ballio, J P Blein, M R Fullone, M Rossignol and R Scalla


Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC, both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the fluorescence quenching of the dye 9-amino-6-chloro-2-methoxyacridine, markedly increased upon addition of fusicoccin to the reconstituted system. This effect could not be observed when binding sites and ATPase preparations were separately reconstituted into the proteoliposomes, thus demonstrating that fusicoccin binding to its receptor is a prerequisite for ATPase stimulation

Topics: Research Article
Year: 1988
OAI identifier:
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.