Location of Repository

ACTION OF HAEMOPHILUS CULTURES ON δ-AMINOLEVULINIC ACID

By Ernst L. Biberstein, Patricia D. Mini and Marjorie G. Gills

Abstract

Biberstein, Ernst L. (University of California, Davis), Patricia D. Mini, and Marjorie G. Gills. Action of Haemophilus cultures on δ-aminolevulinic acid. J. Bacteriol. 86:814–819. 1963.—Utilization of δ-aminolevulinic acid (ALA) by strains of Haemophilus recovered from various sources was investigated. With the 37 cultures studied, there was perfect correlation between absence of hemin requirement and ability to convert ALA to porphyrin. A total of 29 strains, including representatives of H. parainfluenzae, H. parahaemolyticus, H. “parasuis,” and H. gallinarum, fell into this group. The remaining eight isolates, which were incapable of porphyrin synthesis from ALA, were strains of H. influenzae, H. suis, and a Haemophilus culture of uncertain classification obtained from a splenic abscess in a deer. In the active preparations, the products of synthesis included a mixture of porphyrins, porphobilinogen (PBG), and a pigment which absorbed light strongly at 470 to 480 mμ. Paper chromatographic studies of fractions of supernatants and cells revealed the presence of porphyrins having RF values similar to those of uro- and protoporphyrins, as well as some intermediate rates of migration probably representing coproporphyrins. Porphyrins were found both intra- and extracellularly, and PBG and the pigment absorbing at 470 to 480 mμ were confined to the supernatant

Topics: Articles
Year: 1963
OAI identifier: oai:pubmedcentral.nih.gov:278519
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefor we are unable to provide a PDF.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.