Skip to main content
Article thumbnail
Location of Repository

Increased microbial yield from continuous ambulatory peritoneal dialysis peritonitis effluent after chemical or physical disruption of phagocytes.

By P C Taylor, L A Poole-Warren and R E Grundy


The laboratory diagnosis of continuous ambulatory peritoneal dialysis-associated peritonitis is often hindered by either the absence of or the recovery of low numbers of viable microorganisms. This may be the result of sequestration of bacteria within phagocytes. Sonication of clinical specimens prior to culturing or culturing on saponin-containing media resulted in the growth of significantly greater numbers of colonies than standard culturing on conventional media. In addition, the demonstration that microorganisms are sequestered in phagocytes helped to establish the pathogenic nature of such isolates and distinguish them from contaminants even when present in low numbers. A variety of physical and chemical techniques can disrupt phagocytes and improve the sensitivity of laboratory methods used to confirm the diagnosis of peritonitis in continuous ambulatory peritoneal dialysis patients

Topics: Research Article
Year: 1987
OAI identifier:
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.