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Increased detection of human immunodeficiency virus antigenemia after dissociation of immune complexes at low pH.

By R A Pokriefka, O Manzor, N P Markowitz, L D Saravolatz, P Kvale and R M Donovan


This study compared the number of patients with detectable human immunodeficiency virus (HIV) antigenemia after immune complex (IC) dissociation by established methods using either 0.5 NCl or 1.5 M glycine buffer. Without IC dissociation, HIV antigen was detected in 43% of patients. After dissociation, the HCl method detected only an additional 7% of patients (P = 0.09), while the glycine method detected an additional 34% (P < 0.001). However, care must be taken in setting the threshold of the standards, and confirmatory neutralization assays should be performed to ensure specificity of HIV antigen enzyme immunoassay after IC dissociation

Topics: Research Article
Year: 1993
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Provided by: PubMed Central
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