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Involvement of Protein and Ribonucleic Acid in the Association of Deoxyribonucleic Acid with Other Cell Components of Escherichia coli

By Bruce Porter and Dorothy K. Fraser

Abstract

Cells of Escherichia coli were labeled with precursors of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein, lysed with detergent, and examined by starch-block electrophoresis and CsCl density gradient centrifugation. A large amount of the DNA was seen to remain at positions of low electrophoretic mobility and light density along with tryptophan and arginine-containing proteins and some RNA. Addition of labeled, phenol-extracted DNA to unlabeled cells prior to lysis and electrophoresis showed that only a small amount of the DNA became associated during or after lysis. Sonic treatment of a lysate removed most of the DNA to a position of electrophoretic mobility and density similar to that of free DNA, whereas pronase and ribonuclease released only a part of the DNA. We concluded that binding of DNA to cell membranes or other cell components occurs in the cell prior to lysis and involves protein and probably a specific type of RNA

Topics: Genetics and Molecular Biology
Year: 1968
OAI identifier: oai:pubmedcentral.nih.gov:252258
Provided by: PubMed Central
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