Use of Neomycin in the Isolation of Mutants Blocked in Energy Conservation in Escherichia coli
A neomycin-resistant mutant of Escherichia coli K-12 unable to grow on Krebs cycle intermediates has been isolated. The mutant retained its respiratory capacity, but lacked membrane Mg2+-Ca2+-stimulated adenosine triphosphatase activity (EC 22.214.171.124)
Physiology and Metabolism
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