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Deoxyribonucleic Acid Homology and Relative Genome Size in Mycoplasma

By Anne M. Peterson and Mary E. Pollock

Abstract

The deoxyribonucleic acid homologies of Mycoplasma laidlawii type A and type B, M. pulmonis (#47 and #63), and M. hominis were determined by membrane methodology. The homology data revealed a difference in genome size between M. laidlawii type A and type B. This difference also held with stringent conditions of annealing (high temperature). Little or negligible homology was shown to exist between the M. laidlawii strains type A and type B and M. pulmonis strains 47 and 63 and M. hominis. M. hominis showed less than 10% homology to the M. pulmonis and M. laidlawii strains. Neither of the M. laidlawii strains showed more than 2% annealing to the M. pulmonis strains. Reaction rate studies are suggested as a means of demonstrating the phylogenetic relationship between the Mycoplasma and other microorganisms

Topics: Taxonomy, Ecology, and Morphology and Structure
Year: 1969
OAI identifier: oai:pubmedcentral.nih.gov:250074
Provided by: PubMed Central
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