Skip to main content
Article thumbnail
Location of Repository

Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

By T L Sladek and J W Jacobberger

Abstract

Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies

Topics: Research Article
Year: 1990
OAI identifier: oai:pubmedcentral.nih.gov:249510
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.