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Lack of evidence for a saturable tetracycline transport system in Staphylococcus aureus.

By I Chopra, S Ismail and B Oliva


Previous studies on tetracycline transport into Staphylococcus aureus identified a high-affinity, saturable uptake system for the antibiotic (Km, 4.76 microM) (B.L. Hutchings, Biochim. Biophys. Acta 174:734-738, 1969). However, the earlier results could not be confirmed using conditions that permitted energy-dependent, concentrative uptake of tetracycline. Kinetic artifacts introduced by inappropriate washing procedures may explain the previous results

Topics: Research Article
Year: 1991
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Provided by: PubMed Central
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