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Properties of Alginate Lyases from Marine Bacteria

By R. Scott Doubet and Ralph S. Quatrano

Abstract

Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes

Topics: Physiology and Biotechnology
Year: 1984
OAI identifier: oai:pubmedcentral.nih.gov:239751
Provided by: PubMed Central
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