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A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases.

By A L Passarelli, J W Todd and L K Miller

Abstract

We have identified and sequenced a novel baculovirus gene, late expression factor eight gene (lef-8), of Autographa californica nuclear polyhedrosis virus that is necessary for efficient expression from late and very late virus gene promoters in a transient expression assay. The predicted gene product, LEF-8, has a molecular mass of 102 kDa and contains a conserved sequence motif, GXKX4HGQ/NKG, found in DNA-directed RNA polymerases throughout the animal, plant, and microbial kingdoms

Topics: Research Article
Year: 1994
OAI identifier: oai:pubmedcentral.nih.gov:236397
Provided by: PubMed Central
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