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Selection of yeast auxotrophs by thymidylate starvation.

By B J Barclay and J G Little

Abstract

A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists

Topics: Research Article
Year: 1977
OAI identifier: oai:pubmedcentral.nih.gov:235609
Provided by: PubMed Central
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