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Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.

By M S Guyer, D Figurski and N Davidson

Abstract

pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67

Topics: Research Article
Year: 1976
OAI identifier: oai:pubmedcentral.nih.gov:233009
Provided by: PubMed Central
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