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Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.

By G Boivin, J Handfield, G Murray, E Toma, R Lalonde, J G Lazar and M G Bergeron


We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS

Topics: Research Article
Year: 1997
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Provided by: PubMed Central
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