Phenol Oxidase

Abstract

Phenol oxidase is an enzyme which is responsible for hydroxylation of phenols and dehydrogenation of O. diphenols into quinone. It is known to be distributed in the blood as well as in the cuticle of crustaceans (Summers, 1967). The enzyme can be assayed either spectrophotometrically, by measuring the dopa chrome formed (Preston & Taylor, 1970) or monometrically by measuring the oxygen. consumed during the oxidation of the substrates (Hackman & Goldberg, 1967). The spectrophotometry method of assaying the enzyme of blood from Scylla serrata is presented here