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Cloning of immunity and structural genes for colicin V.

By K K Frick, R L Quackenbush and J Konisky

Abstract

The colicin V immunity and structural genes of plasmid pColV-B188 were cloned into the vectors pMB9, pBR322, and pMK16. Both genes are closely linked and can be isolated on a 900-base-pair deoxyribonucleic acid fragment. Insertion of the transposon Tn5 into this cloned sequence led to the construction of a mutant plasmid which conferred colicin V immunity, but not the ability to produce this colicin. Analysis of the products determined by these cloned genes in cells has led to the conclusion that the polypeptide involved in immunity has a molecular weight of about 6,500, whereas the colicin has a molecular weight of approximately 4,000

Topics: Research Article
Year: 1981
OAI identifier: oai:pubmedcentral.nih.gov:216232
Provided by: PubMed Central
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