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Elemental composition of bacterial metachromatic inclusions determined by electron microprobe X-ray analysis.

By J A Webster, D D Fay, J L Costa, P M Jones and R Hugh


Electron microscopy and microprobe X-ray analysis were used to study metachromatic inclusions of Spirillum itersonii , Corynebacterium diphtheriae, and Micrococcus luteus. In situ metachromatic inclusions were electron dense and contained phosphorus and divalent cations. Metachromatic inclusions isolated by anion-exchange column chromatography and by isoosmolar Metrizamide density gradient centrifugation were similar in composition to in situ inclusions

Topics: Research Article
Year: 1984
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Provided by: PubMed Central
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