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Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.

By F Kawamura and R H Doi


A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development

Topics: Research Article
Year: 1984
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Provided by: PubMed Central
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