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Spontaneous deletion of a 20-kilobase DNA segment carrying genes specifying isopropylbenzene metabolism in Pseudomonas putida RE204.

By R W Eaton and K N Timmis

Abstract

The genes encoding isopropylbenzene metabolism in Pseudomonas putida RE204 are readily lost in two ways: by loss (curing) of plasmid pRE4 which specifies the catabolic pathway and by deletion from pRE4 of an approximately 20-kilobase segment of DNA carrying the catabolic genes. The presence of DNA sequences at the ends of the catabolic gene region sharing homology with one another suggests that the deletions result from recombination events between these homologous sequences

Topics: Research Article
Year: 1986
DOI identifier: 10.1128/jb.168.1.428-430.1986
OAI identifier: oai:pubmedcentral.nih.gov:213470
Provided by: PubMed Central
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