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Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.

By A Diolez, F Richaud and A Coleno


The pelB gene, which encodes one of the five pectate lyase isoenzymes of Erwinia chrysanthemi 3937, was mutagenized with a mini-Mu transposable element that can form gene fusions to the neomycin phosphotransferase-encoding region. Secondary mutants resistant to kanamycin in the absence of polygalacturonate, an inducer of wild-type pectate lyase activities, were selected. Such mutants produced other pectate lyase isoenzymes in the absence of the inducer

Topics: Research Article
Year: 1986
DOI identifier: 10.1128/jb.167.1.400-403.1986
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Provided by: PubMed Central
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