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Apurinic endonucleases from Saccharomyces cerevisiae also recognize urea residues in oxidized DNA.

By C C Chang, Y W Kow and S S Wallace

Abstract

Saccharomyces cerevisiae apurinic endonucleases E cochromatographed with activity against a DNA substrate containing urea residues. The urea-recognizing activity of endonuclease E was competitively inhibited by apurinic DNA, and the heat labilities of both activities were the same. The apparent VmaxS of endonuclease E for both substrates were about the same, while the apparent Km for urea-containing DNA was about threefold greater than that for apurinic DNA. These results were similar to those obtained previously with Escherichia coli exonuclease III (Y. Kow and S. Wallace, Proc. Natl. Acad. Sci. USA 82:8354-8358, 1985) and suggest that the ability to recognize urea residues may be a general property of apurinic endonucleases

Topics: Research Article
Year: 1987
OAI identifier: oai:pubmedcentral.nih.gov:211750
Provided by: PubMed Central
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