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Transformation of a filamentous cyanobacterium by electroporation.

By T Thiel and H Poo

Abstract

The filamentous cyanobacterium Anabaena sp. strain M131 was transformed with the shuttle vector pRL6 by electroporation. Optimum conditions for electroporation required relatively high field strengths with short time constants. Restriction significantly lowered the efficiency of transformation. A plasmid containing a single unmodified AvaII restriction site transformed cells with about 100-fold-lower efficiency than did the same plasmid with a modified restriction site

Topics: Research Article
Year: 1989
OAI identifier: oai:pubmedcentral.nih.gov:210424
Provided by: PubMed Central
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