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Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

By S Tabata, A Higashitani, M Takanami, K Akiyama, Y Kohara, Y Nishimura, A Nishimura, S Yasuda and Y Hirota


A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli

Topics: Research Article
Year: 1989
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Provided by: PubMed Central
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